Yesterday, I performed my mock assay and I messed up my estradiol saliva assay.
Here’s a list of all the things I did wrong 😭😅
- Had too many air bubbles in my pipette
- I pipetted a saliva sample into a well that was supposed to be empty
- I didn’t fill up my estradiol standards properly (I didn’t put enough assay dilute)
- I didn’t place my plate in the plate wash securely
Ahhhh and then my R^2 value came out to like 0.842, but when “corrected,” it came out to like 0.869 (you’re supposed to have an R^2 value of 1 or 0.999).
So yea, it went pretty poorly. But I appreciate that Scott Le (who was supervising me) was super patient with me, and took the time to explain the process step by step.
Oh also, shoutout to Lotte van Dammen as well! She was the one who got me started with the wet lab.
Overall, it was a good learning experience. For the next assay, I’ll re-read the protocols for assaying different biomarkers and make sure I understand them better.